Wetland mesocosms were constructed to assess two hybrid poplar clones (Populustrichocarpa x P. deltoides x P. nigra '345-1' and '347-14') for treating saline water high in boron (B) and selenium (Se), and a hydroponic experiment was performed to test the B tolerance and B accumulation in both clones. In the mesocosm experiment, clone 345-1 exhibited no toxic symptoms at an EC of 10 mS cm(-1), while clone 347-14 showed slight toxic symptoms at 7.5 mS cm-1. The removal percentages of B, Se, sodium (Na), and chloride (Cl) ranged from 26.7-45.6%, 50-69.4%, 18.4-24.0%, and 15.8-23.2%, respectively, by clone 345-1, and from 22.9-29.4%, 31.7-43.8%, 16.5-24.2%, and 14.9-23.9%, respectively, by clone 347-1. In the hydroponic experiment, B toxic symptoms were observed at treatments of 150 and 200 mg B L-1 for clones 345-1 and 347-14, respectively. The greatest leaf B concentrations of 3699 and 1913 mg kg(-1) were found in clone 345-1 and clone 347-14, respectively. The translocation factor (TF) of clone 347-14 was less than clone 345-1. Clone 345-1 only showed significantly greater (P < 0.05) B removal percentages than clone 347-14 when B treatment was <20 mg B L-1. In conclusion, both tested poplar clones competitively accumulated and removed B and Se in constructed wetlands. (C) 2017 Published by Elsevier B.V.